Buffer Solution Preparation Calculator for His-Asp

The preparation of His-Asp buffer solutions is essential in biochemical experiments requiring precise pH control, especially around physiological pH levels.

Historical Background

Histidine-Aspartate (His-Asp) buffer systems are widely used in enzymatic studies and protein chemistry. These buffers are chosen for their ability to maintain a specific pH range and to stabilize sensitive biomolecules under experimental conditions.

Calculation Formula

To prepare a buffer solution:

1. Volume of stock solution required:
   \[ \text{Volume of stock solution} = \frac{\text{Desired molarity} \times \text{Desired volume}}{\text{Stock solution molarity}} \]
2. Amount of compound required:
   \[ \text{Compound amount (g)} = \text{Desired molarity} \times \text{Desired volume (L)} \times \text{Molecular weight (g/mol)} \]

Example Calculation

To prepare a 10 mM His-Asp buffer at pH 6.0 with a total volume of 1 L using a 1 M stock solution:

1. Volume of stock solution needed:
   \[ \text{Volume of stock solution} = \frac{10 \text{ mM} \times 1 \text{ L}}{1000 \text{ mM}} = 0.01 \text{ L} = 10 \text{ mL} \]

2. Amount of His-Asp compound needed (assuming molecular weight = 150 g/mol):
   \[ \text{Compound amount} = 10 \times 10^{-3} \text{ mol/L} \times 1 \text{ L} \times 150 \text{ g/mol} = 1.5 \text{ g} \]

Importance and Usage Scenarios

This buffer is critical for enzymatic reactions, protein folding studies, and maintaining the stability of biological samples during experiments. His-Asp buffers ensure the pH remains stable, even when biological reactions produce or consume hydrogen ions.

Common FAQs

1. What is the molecular weight of His-Asp?

   • The molecular weight of Histidine-Aspartate (His-Asp) varies depending on the formulation but is generally around 150 g/mol.

2. How do I adjust the pH?

   • Adjust the pH by adding small amounts of NaOH or HCl to reach the desired level.

3. What are typical applications of His-Asp buffers?

   • His-Asp buffers are used in enzymatic studies, protein stability assays, and experiments requiring stable pH environments.

This tool simplifies buffer preparation, providing researchers with an efficient method to create solutions tailored to their experimental needs.