Primer Dilution Calculator

A primer dilution calculator is essential for accurately preparing primers for PCR or other molecular biology experiments. It determines the volumes of a concentrated stock solution and diluent required to achieve a desired concentration.

Key Concepts

1. Initial Concentration (µM): The concentration of your current stock primer solution.
2. Desired Concentration (µM): The target concentration for your diluted primer.
3. Final Volume (µL): The total volume you need for your final solution.

Formula Used

To calculate the volume of the stock solution needed:

\[ \text{Volume of Stock Solution (µL)} = \frac{\text{Desired Concentration (µM)} \times \text{Final Volume (µL)}}{\text{Initial Concentration (µM)}} \]

The diluent volume is calculated as:

\[ \text{Volume of Diluent (µL)} = \text{Final Volume (µL)} - \text{Volume of Stock Solution (µL)} \]

Example Calculation

For an initial concentration of 100 µM, a desired concentration of 10 µM, and a final volume of 100 µL:

\[ \text{Volume of Stock Solution (µL)} = \frac{10 \times 100}{100} = 10 \text{ µL} \]

\[ \text{Volume of Diluent (µL)} = 100 - 10 = 90 \text{ µL} \]

This helps ensure accurate primer preparations for your experiments.