Protein Molarity Calculator
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The Protein Molarity Calculator is used to determine the molar concentration (Molarity) of a protein solution based on the mass of the protein, its molecular weight, and the volume of the solution. Molarity is a key parameter in biochemistry and molecular biology for quantifying the concentration of proteins in experimental solutions.
Calculation Formula
The molarity (M) of a protein solution is calculated as:
\[ \text{Molarity (M)} = \frac{\text{Protein Mass (g)}}{\text{Molecular Weight (g/mol)} \times \text{Solution Volume (L)}} \]
Example Calculation
If you have 50 mg of a protein with a molecular weight of 50,000 g/mol in a 2 mL solution:
- Convert mass to grams: 50 mg = 0.05 g.
- Convert volume to liters: 2 mL = 0.002 L.
- Calculate molarity:
\[ \text{Molarity} = \frac{0.05 \text{ g}}{50,000 \text{ g/mol} \times 0.002 \text{ L}} = 5 \times 10^{-4} \text{ M} \]
Importance and Usage Scenarios
This calculator is essential for researchers who need precise protein concentrations for experiments such as enzyme kinetics, binding assays, and structural studies. By knowing the molarity, one can accurately control the amount of protein added to reactions or treatments in scientific experiments.